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Journal: Nature communications

Article Title: Graded heterogeneity of metabotropic signaling underlies a continuum of cell-intrinsic temporal responses in unipolar brush cells.

doi: 10.1038/s41467-021-22893-8

Figure Lengend Snippet: Fig. 2 Continuous variations in molecular and functional properties of UBCs. a Schematics of cerebellar circuit. b UMAP embedding showing normalized expression of gene involved in mGluR1 signaling cascade. c Same as in b for AMPA and NMDA receptors. d Same as in b for genes involved in mGluR2/ 3 signaling cascade. e Example spiking responses in different UBCs to a burst of MF input (20 stimuli at 100 Hz). f Instantaneous firing rate for the same cells.

Article Snippet: Sequential drug wash-ins were performed using a computercontrolled solenoid manifold system (ValveLink 8.2, Automate Scientific, Inc., Berkeley, CA) with a flow rate of 1 to 2 mL/min, where indicated, with mGluR1 antagonist (100 μM LY357385, Tocris Bio-Techne, Minneapolis, MN), AMPA receptor blocker (5 μM NBQX), NMDA receptor blocker (2 μM R-CPP), mGluR2/ 3 antagonist (1 μM LY341495, Tocris Bio-Techne, Minneapolis, MN) and DGK inhibitor II (100 μM R59949, MilliporeSigma, St. Louis, MO) One-photon glutamate uncaging.

Techniques: Functional Assay, Expressing

Journal: Nature communications

Article Title: Graded heterogeneity of metabotropic signaling underlies a continuum of cell-intrinsic temporal responses in unipolar brush cells.

doi: 10.1038/s41467-021-22893-8

Figure Lengend Snippet: Fig. 5 mGluR1 and mGluR2/3-mediated synaptic currents determine the spiking kinetics. a Sample cell-attached recording (top) instantaneous firing rate (middle) and synaptic current measured with whole-cell voltage clamp (bottom) in a cell with fast response. b Same as in a but for a cell with intermediate speed response. c Same as in a but for a cell with clear biphasic synaptic current (bottom). d Same as in a but for a cell with slow biphasic response. e Same as in a but for a cell with only a pause in firing. f Half-decay times of firing rates vs. half-decay times of currents and linear fit on a log-log plot (Radj2 = 0.91, slope = 0.88, intercept = −0.22, n = 17). g Peak firing rate vs. peak current amplitude and linear fit on a log-log plot (Radj2 = 0.69, slope = 0.71, intercept = −0.65, n = 17). h Pause duration vs. amplitude of the current at stimulation offset, and linear fit with log10 response variable (black, Radj2 = 0.44, slope = 0.01, intercept = −0.42, n = 20). i Heatmap of peak normalized mGluR1-mediated current (n = 10). j Heatmap of peak normalized mGluR2/3-mediated current (n = 7). k Average synaptic response before (black), after (red) mGluR1 antagonist, and after AMPA/NMDA receptor antagonist wash-ins (gray). Each trace is an average of 8 trials. l Average synaptic response before (top, black) and after (top, blue) mGluR2/3 antagonist wash-in, and their difference (bottom, black). Each trace is an average of 8 trials.

Article Snippet: Sequential drug wash-ins were performed using a computercontrolled solenoid manifold system (ValveLink 8.2, Automate Scientific, Inc., Berkeley, CA) with a flow rate of 1 to 2 mL/min, where indicated, with mGluR1 antagonist (100 μM LY357385, Tocris Bio-Techne, Minneapolis, MN), AMPA receptor blocker (5 μM NBQX), NMDA receptor blocker (2 μM R-CPP), mGluR2/ 3 antagonist (1 μM LY341495, Tocris Bio-Techne, Minneapolis, MN) and DGK inhibitor II (100 μM R59949, MilliporeSigma, St. Louis, MO) One-photon glutamate uncaging.

Techniques: